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Journal / Cancer Research / 淋巴结慢病毒北疆-转基因 Organoids…
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Cancer Research

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JoVE Journal Cancer Research
淋巴结慢病毒北疆-转基因 Organoids 培养的大鼠结肠肿瘤的高灵敏度检测
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淋巴结慢病毒北疆-转基因 Organoids 培养的大鼠结肠肿瘤的高灵敏度检测

Article DOI: 10.3791/57374-v • 09:16 min • June 14th, 2018
June 14th, 2018 •
1,2, 1,2, 2,4, 5, 1, 1, 1, 3, 2, 1, 2
1Department of Coloproctological Surgery, Juntendo University Faculty of Medicine, 2Department of Molecular Pathogenesis, Juntendo University Faculty of Medicine, 3Atopy Research Center, Juntendo University Faculty of Medicine, 4Department of Oral Pathobiological Science and Surgery, Tokyo Dental College, 5Department of Gastroenterology, Juntendo University Faculty of Medicine

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为了允许高度敏感的检测传播的人大肠癌 (CRC) 细胞的殖民组织, 我们在此显示了一个协议, 以有效地转导绿色荧光蛋白 (GFP) 慢病毒载体粒子到 PDX 衍生 CRC organoid 细胞在注射到受体小鼠之前, 用立体荧光显微镜观察。

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癌症研究 问题 136 人 CRC 患者源性肿瘤移植 (PDXs) GFP 标记的 PDX 衍生肿瘤 organoids 慢病毒载体转导 微转移 3D 培养
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