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Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord
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Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord

Article DOI: 10.3791/57382-v • 08:41 min • May 12th, 2018
May 12th, 2018 •
*1,2, *1, 1, 1
1Institute of Pharmacology and Toxicology, University of Zurich, 2Institute of Pharmaceutical Sciences, Swiss Federal Institute (ETH) Zurich
* These authors contributed equally

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Summary
Automatic Translation

Intraspinal injection of recombinase dependent recombinant adeno-associated virus (rAAV) can be used to manipulate any genetically labelled cell type in the spinal cord. Here we describe how to transduce neurons in the dorsal horn of the lumbar spinal cord. This technique enables functional interrogation of the manipulated neuron subtype.

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Adeno-associated Virus Transgene Expression Genetically Defined Neurons Spinal Cord Intraspinal Delivery Recombinant Adeno-associated Viruses Histological Labeling Functional Manipulation Pain Field Neuronal Circuits Perception Of Pain Modalities Genetically Labeled Neurons Temporally Controlled Fashion Caudal Rib Pair Palpating Vertebral Column Longitudinal Cut Skin Detachment Surgical Procedure Sodium Chloride Solution Anatomical Landmarks Vertebrae Targeting
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