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JoVE Journal Neuroscience
Expressão do Transgene de mediada por vírus adeno-associado em geneticamente definidos neurônios da medula espinhal
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Expressão do Transgene de mediada por vírus adeno-associado em geneticamente definidos neurônios da medula espinhal

Article DOI: 10.3791/57382-v • 08:41 min • May 12th, 2018
May 12th, 2018 •
*1,2, *1, 1, 1
1Institute of Pharmacology and Toxicology, University of Zurich, 2Institute of Pharmaceutical Sciences, Swiss Federal Institute (ETH) Zurich
* These authors contributed equally

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Intraspinal injeção de recombinase dependente recombinante vírus adeno-associado (rAAV) pode ser usada para manipular qualquer tipo de célula geneticamente rotulados na medula espinhal. Aqui descrevemos como transduce neurônios no Corno dorsal da medula espinhal lombar. Esta técnica permite interrogatório funcional do subtipo neurônio manipulado.

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Neurociência questão 135 neurônios da coluna vertebral medula espinhal corno Dorsal vírus Adeno-associado recombinante AAV sistema sensorial injeção intra-espinhal Cre-LoxP sistema
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