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Journal / Neuroscience / Adeno-associeret Virus-medieret transgen udtryk i …
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Neuroscience

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JoVE Journal Neuroscience
Adeno-associeret Virus-medieret transgen udtryk i genetisk definerede neuroner i rygmarven
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Adeno-associeret Virus-medieret transgen udtryk i genetisk definerede neuroner i rygmarven

Article DOI: 10.3791/57382-v • 08:41 min • May 12th, 2018
May 12th, 2018 •
*1,2, *1, 1, 1
1Institute of Pharmacology and Toxicology, University of Zurich, 2Institute of Pharmaceutical Sciences, Swiss Federal Institute (ETH) Zurich
* These authors contributed equally

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Intraspinal injektion af recombinase afhænger af rekombinant adeno-associeret virus (rAAV) kan bruges til at manipulere nogen genetisk mærket celletype i rygmarven. Her beskriver vi, hvordan du transduce neuroner i dorsal Hornet af lumbal rygmarven. Denne teknik gør det muligt for funktionelle forhør af undertypen manipulerede neuron.

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Neurovidenskab spørgsmålet 135 Spinal neuroner rygmarven dorsale Horn rekombinant Adeno-associeret Virus AAV sensorisk System Intraspinal injektion Cre-LoxP System
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