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Journal / Cancer Research / Isolering af menneskelige endotelceller fra…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Isolering af menneskelige endotelceller fra normale kolon og Colorectal karcinom - en forbedret protokol
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Isolering af menneskelige endotelceller fra normale kolon og Colorectal karcinom - en forbedret protokol

Article DOI: 10.3791/57400-v • 10:54 min • April 4th, 2018
April 4th, 2018 •
1, 1, 1, 2, 3, 4, 4, 2, *2, *1
1Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 2Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 3Division of Nephropathology, Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 4Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg
* These authors contributed equally

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Tumor endotelceller er vigtige determinanter af tumor mikromiljø og forløbet af sygdommen. Her, er en protokol til isolering af ren og levedygtige endotelceller fra menneskelige colorectal karcinom og normal kolon skal anvendes i drug test og patogenese forskning beskrevet.

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Kræftforskning spørgsmål 134 endotelceller fartøj Colorectal karcinom Tumor Normal kolon CD31 VE-cadherin CD105 FACS-sortering væv Dissociation Tumor mikromiljø fibroblaster
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