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JoVE Journal Cancer Research
Isolement des cellules endothéliales humaines du côlon Normal et carcinome Colorectal - un protocole amélioré
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Isolement des cellules endothéliales humaines du côlon Normal et carcinome Colorectal - un protocole amélioré

Article DOI: 10.3791/57400-v • 10:54 min • April 4th, 2018
April 4th, 2018 •
1, 1, 1, 2, 3, 4, 4, 2, *2, *1
1Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 2Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 3Division of Nephropathology, Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 4Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg
* These authors contributed equally

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Cellules endothéliales tumorales sont des déterminants importants du microenvironnement tumoral et l’évolution de la maladie. Ici, un protocole pour l’isolement des cellules endothéliales pures et viables de carcinome colorectal humain et côlon normal pour être utilisé dans la recherche de tests et la pathogenèse est décrite.

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Recherche sur le cancer numéro 134 cellules endothéliales navire carcinome Colorectal tumeur côlon Normal CD31 VE-cadhérine CD105 tri des FACS Dissociation tissulaire microenvironnement tumoral fibroblastes
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