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Journal / Cancer Research / Isolamento di cellule endoteliali umane da Colon…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Isolamento di cellule endoteliali umane da Colon normale e Carcinoma colorettale - un protocollo migliorato
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Isolamento di cellule endoteliali umane da Colon normale e Carcinoma colorettale - un protocollo migliorato

Article DOI: 10.3791/57400-v • 10:54 min • April 4th, 2018
April 4th, 2018 •
1, 1, 1, 2, 3, 4, 4, 2, *2, *1
1Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 2Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 3Division of Nephropathology, Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 4Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg
* These authors contributed equally

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Cellule endoteliali tumorali sono fattori determinanti importanti del microambiente tumorale e il decorso della malattia. Qui, un protocollo per l'isolamento di cellule endoteliali pure e praticabile da carcinoma colorettale umano e colon normale a essere impiegata nella ricerca di droga test e la patogenesi è descritto.

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Ricerca sul cancro emissione 134 cellule endoteliali nave Carcinoma del colon-retto tumore Colon normale CD31 VE-caderina CD105 FACS-ordinamento tessuto dissociazione microambiente tumorale fibroblasti
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