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疫苗开发中佐剂细胞毒性 T 淋巴细胞生成能力的快速体内评价
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Rapid In Vivo Assessment of Adjuvant’s Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development
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疫苗开发中佐剂细胞毒性 T 淋巴细胞生成能力的快速体内评价

DOI:
10.3791/57401-v

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09:03 min

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June 19, 2018

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, , , , , , ,
1Department of Vaccinology and Applied Microbiology,Helmholtz Centre for Infection Research

Chapters

  • 00:05Title
  • 01:03OT-I T Cell Staining and Adoptive Transfer
  • 04:22Endo-Free OVA +/- Adjuvant Immunization and Lymphocyte Isolation
  • 05:21Flow Cytometry
  • 07:30Results: Representative Adjuvant Drive Cytotoxic T Cell Generation
  • 08:31Conclusion

Summary

Automatic Translation

Automatic Translation

我们在这里提出一个标准免疫技术 (CFSE 染色的应用), 旨在快速监测佐剂介导的细胞毒性 T 淋巴细胞 (CTL) 生成在体内.这种对 CTL 容量的快速估计有助于开发预防性疫苗, 防止细胞内病原体和治疗性癌症疫苗的发展。

Tags

OT1 CD8 T CellsCFSEAdoptive TransferAdjuvantCytotoxic T Lymphocyte (CTL) ResponseVaccine DevelopmentLymph NodeSpleenMagnetic Bead Isolation

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