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Genetics

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JoVE Journal Genetics
Undersøgelse af Protein rekruttering til DNA læsioner ved hjælp af 405 Nm Laser mikro-bestråling
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Undersøgelse af Protein rekruttering til DNA læsioner ved hjælp af 405 Nm Laser mikro-bestråling

Article DOI: 10.3791/57410-v • 12:29 min • March 20th, 2018
March 20th, 2018 •
*1, *1, *1, 1, 1, 1
1Department of Biology, Université de Sherbrooke
* These authors contributed equally

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At studere DNA skader reparation kinetik kræver et system til at fremkalde læsioner på definerede sub nukleare regioner. Vi beskriver en metode til at oprette lokaliserede dobbelt-strenget pauser ved hjælp af en laser-scanning Konfokal mikroskop udstyret med en 405 nm laser og give automatiserede procedurer for at kvantificere dynamikken i reparation faktorer på disse læsioner.

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Genetik sag 133 DNA-skader dobbelt-strenget DNA pauser mikro-bestråling Konfokal mikroskopi immunofluorescens real-time imaging automatiseret billedanalyse
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