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JoVE Journal Genetics
Utredning av Protein rekrytering till DNA lesioner med 405 Nm Laser mikro-bestrålning
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Utredning av Protein rekrytering till DNA lesioner med 405 Nm Laser mikro-bestrålning

Article DOI: 10.3791/57410-v • 12:29 min • March 20th, 2018
March 20th, 2018 •
*1, *1, *1, 1, 1, 1
1Department of Biology, Université de Sherbrooke
* These authors contributed equally

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Studera DNA skador reparation kinetik kräver ett system att inducera lesioner vid definierade sub nukleära regioner. Vi beskriver en metod för att skapa lokaliserade dubbelsträngat raster med laserskanning confocal Mikroskop utrustad med en 405 nm laser och tillhandahålla automatiserade procedurer för att kvantifiera dynamiken i reparation faktorer vid dessa skador.

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Genetik fråga 133 DNA-skador dubbelsträngat DNA raster mikro-bestrålning confocal microscopy immunofluorescens realtid imaging automatiserad bildanalys
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