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JoVE Journal Developmental Biology
Bedömningen av lysosomala alkalisering i tarmen av Live Caenorhabditis elegans
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Bedömningen av lysosomala alkalisering i tarmen av Live Caenorhabditis elegans

Article DOI: 10.3791/57414-v • 06:18 min • April 13th, 2018
April 13th, 2018 •
1, 1
1Department of Biology, University of Saskatchewan

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En steg för steg guide till probe förlust av lysosomala surhet i inälvan av C. elegans med det pH-känsliga vitala färgämnet 5 (6) - karboxi - 2', 7'-dichlorofluorescein diacetat (cDCFDA)

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Utvecklingsbiologi fråga 134 C. elegans lysosomer pH v-ATPas proteinkatabolism cDCFDA
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