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Journal / Immunology and Infection / Development and Validation of an Ultrasensitive…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
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Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Article DOI: 10.3791/57421-v • 08:26 min • June 14th, 2018
June 14th, 2018 •
*1,2, *1,2, 3, 4, 3,5, 1,2
1Immunobiology of Dendritic Cells, Institut Pasteur, 2INSERM U1223, 3Laboratory of Neurogenetics and Neuroinflammation, INSERM UMR1163, Institut Imagine, 4MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, 5Manchester Centre for Genomic Medicine, University of Manchester
* These authors contributed equally

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Summary
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Here we present a protocol to describe the development and validation of a single molecule array digital ELISA assay, which enables the ultra-sensitive detection of all IFN-α subtypes in human samples.

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Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay Human Interferon-alpha Nature Of Interferon Induced Responses Regulation Of Interferon Induced Responses Biological Impact Of Interferon Induced Responses Auto-immunity Infection Biomarker Discovery Patient Management Cytokines Sensitivity Autoimmune Polyendocrine System Type One Paramagnetic Beads Microfuge Tube Magnetic Separator Wash Beads With BWB Wash Beads With BCB Activate Beads EDC
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