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Journal / Immunology and Infection / Utveckling och validering av en Ultrasensitive…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Utveckling och validering av en Ultrasensitive enda molekyl Array Digital Enzyme-linked Immunosorbent Assay för humant Interferon-α
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Utveckling och validering av en Ultrasensitive enda molekyl Array Digital Enzyme-linked Immunosorbent Assay för humant Interferon-α

Article DOI: 10.3791/57421-v • 08:26 min • June 14th, 2018
June 14th, 2018 •
*1,2, *1,2, 3, 4, 3,5, 1,2
1Immunobiology of Dendritic Cells, Institut Pasteur, 2INSERM U1223, 3Laboratory of Neurogenetics and Neuroinflammation, INSERM UMR1163, Institut Imagine, 4MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, 5Manchester Centre for Genomic Medicine, University of Manchester
* These authors contributed equally

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Här presenterar vi ett protokoll för att beskriva utveckling och validering av en enda molekyl array digital ELISA-testet, vilket gör den ultra känslig detektion av alla IFN-α undertyper i mänskliga prover.

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Immunologi och infektion fråga 136 Digital ELISA protein biomarkörer interferon antikroppar och cytokiner hög känslighet
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