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Journal / Chemistry / 透射电镜 hFOB 1.19 和 Saos-2 细胞产生的能量色散 X…
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JoVE Journal Chemistry
透射电镜 hFOB 1.19 和 Saos-2 细胞产生的能量色散 X 射线显微分析
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透射电镜 hFOB 1.19 和 Saos-2 细胞产生的能量色散 X 射线显微分析

Article DOI: 10.3791/57423-v • 14:56 min • June 24th, 2018
June 24th, 2018 •
1, 1, 2,3,4,5,6, 2,3,4,5,6, 1, 1
1Laboratory of Lipid Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 2Université de Lyon, 3Université Lyon 1, 4L'insitut National des Sciences Appliquées (INSA) de Lyon, 5Ecole Supérieure de Chimie Physique Electronique (CPE) Lyon, 6Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR), L'institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS)

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我们提出一个协议, 以比较的矿物质在囊泡释放的两个人骨细胞系: hFOB 1.19 和 Saos-2。利用茜素红 s (AR) 染色、紫外 (紫外) 光可视化、透射电镜 (TEM) 成像和能量色散 X 射线显微分析 (EDX) 对其成矿剖面进行了研究。

Tags

化学 问题 136 矿物质 囊泡 成骨细胞 骨肉瘤 紫外光 透射电镜-EDX
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