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JoVE Journal Chemistry
Analys av mineraler produceras av hFOB 1.19 och Saos-2 celler använder transmissionselektronmikroskopi med Energy Dispersive X-ray mikroanalys
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Analys av mineraler produceras av hFOB 1.19 och Saos-2 celler använder transmissionselektronmikroskopi med Energy Dispersive X-ray mikroanalys

Article DOI: 10.3791/57423-v • 14:56 min • June 24th, 2018
June 24th, 2018 •
1, 1, 2,3,4,5,6, 2,3,4,5,6, 1, 1
1Laboratory of Lipid Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 2Université de Lyon, 3Université Lyon 1, 4L'insitut National des Sciences Appliquées (INSA) de Lyon, 5Ecole Supérieure de Chimie Physique Electronique (CPE) Lyon, 6Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR), L'institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS)

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Vi presenterar ett protokoll för att jämföra staten av mineraler i blåsor släpptes av två mänskliga ben cellinjer: hFOB 1.19 och Saos-2. Sina mineralisering profiler analyserades av Alizarin Red-S (AR-S) färgning, ultraviolett (UV) ljus visualisering, överföring elektronmikroskopi (TEM) imaging och energy dispersive X-ray mikroanalys (EDX).

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