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Journal / Chemistry / Analyse af mineraler produceret af hFOB 1,19 og…
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JoVE Journal Chemistry
Analyse af mineraler produceret af hFOB 1,19 og Saos-2 celler ved hjælp af transmissions elektronmikroskopi med Energy Dispersive X-ray mikroanalyser
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Analyse af mineraler produceret af hFOB 1,19 og Saos-2 celler ved hjælp af transmissions elektronmikroskopi med Energy Dispersive X-ray mikroanalyser

Article DOI: 10.3791/57423-v • 14:56 min • June 24th, 2018
June 24th, 2018 •
1, 1, 2,3,4,5,6, 2,3,4,5,6, 1, 1
1Laboratory of Lipid Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 2Université de Lyon, 3Université Lyon 1, 4L'insitut National des Sciences Appliquées (INSA) de Lyon, 5Ecole Supérieure de Chimie Physique Electronique (CPE) Lyon, 6Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR), L'institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS)

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Vi præsenterer en protokol for at sammenligne tilstanden af mineraler i vesikler udgivet af to menneskelige knogler cellelinjer: hFOB 1,19 og Saos-2. Deres mineralisering profiler blev analyseret af Alizarin rød-S (AR-S) farvning, ultraviolet (UV) lys visualisering, transmissions Elektron Mikroskopi (TEM) billeddannelse og energy dispersive X-ray mikroanalyser (EDX).

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