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Mouse e humanos derivada de cultura de monocamada epitelial gástrica primária para o estudo da regeneração
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Mouse e humanos derivada de cultura de monocamada epitelial gástrica primária para o estudo da regeneração

Article DOI: 10.3791/57435-v • 11:48 min • May 7th, 2018
May 7th, 2018 •
1, 2, 1, 3, 1
1Department of Pharmacology and Systems Physiology, University of Cincinnati, 2Department of Cell & Developmental Biology, University of Michigan, 3Department of Molecular Genetics, Biochemistry & Microbiology, University of Cincinnati

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Aqui descrevemos um protocolo para o estabelecimento e cultivo humano e rato derivada de 3-dimensional organoids gástrica (3D) e o método de transferência de organoids 3D para um monolayer 2-dimensional. O uso da monocamada epitelial gástrica como um romance ensaio de zero-ferida para estudos de regeneração também é descrito.

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Imunologia e infecção ensaio questão 135 zero-ferida organoids gástrica as células-tronco reparação regeneração monocamada epitelial
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