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Bioengineering

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JoVE Journal Bioengineering
Automatiseret dias Scanning og segmentering i Fluorescently mærket væv ved hjælp af en Widefield høj-indhold analysesystem
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Automatiseret dias Scanning og segmentering i Fluorescently mærket væv ved hjælp af en Widefield høj-indhold analysesystem

Article DOI: 10.3791/57440-v • 09:33 min • May 3rd, 2018
May 3rd, 2018 •
1, 2, 1, *1,3, *1
1Department of Clinical Neurosciences, Arnie Charbonneau Cancer Institute, University of Calgary, 2Department of Cell Biology and Anatomy, Hotchkiss Brain Institute, University of Calgary, 3Department of Oncology, Hotchkiss Brain Institute, University of Calgary
* These authors contributed equally

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Her beskriver vi en protokol til den automatiserede segmentering af fluorescently mærket væv på dias ved hjælp af en widefield høj-indhold analysesystem (WHCAS). Denne protokol har omfattende programmer på ethvert område, der involverer kvantitering af fluorescerende markører i biologisk væv, herunder biologiske videnskaber, medicinsk teknik og Sundhedsvidenskab.

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Bioteknologi spørgsmålet 135 automatiseret dias scanning automatiseret segmentering immunohistofluorescence mikroglia makrofager hjernesvulst high-indhold analyse
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