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Journal / Immunology and Infection / Montage und Reinigung der Prototyp schaumig Virus …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Montage und Reinigung der Prototyp schaumig Virus Intasomes
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Montage und Reinigung der Prototyp schaumig Virus Intasomes

Article DOI: 10.3791/57453-v • 10:20 min • March 19th, 2018
March 19th, 2018 •
1, 1, 1
1Department of Cancer Biology and Genetics, The Ohio State University College of Medicine

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Rekombinante Retroviren Integrase und DNA-Oligomere imitiert virale DNA enden können einen enzymatisch aktiven Komplex bekannt als ein Intasome bilden. Intasomes können für biochemische, strukturellen und kinetischen Studien verwendet werden. Dieses Protokoll beschreibt, wie zu montieren und zu reinigen Prototyp schaumig Virus Intasomes.

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Immunologie und Infektion Ausgabe 133 Retrovirus Prototyp schaumig Virus Intasome Integrase Protein komplexe Montage komplexer Proteinreinigung
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