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JoVE Journal
Immunology and Infection

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Forsamling og rensning af Prototype skummende Virus Intasomes
 
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Forsamling og rensning af Prototype skummende Virus Intasomes

Article DOI: 10.3791/57453-v 10:20 min March 19th, 2018
March 19th, 2018

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Summary

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Rekombinant retroviral integrase og DNA oligomerer efterligne viral DNA ender kan danne en enzymatisk aktive kompleks kendt som en intasome. Intasomes kan anvendes til biokemiske, strukturelle og kinetiske studier. Denne protokol detaljer om, hvordan at samle og rense prototype skummende virus intasomes.

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Immunologi og infektion udstede 133 Retrovirus Prototype skummende Virus Intasome Integrase Protein kompleks samling Protein kompleks rensning
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