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Journal / Genetics / Mediada por CRISPR reorganización de la…
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Genetics

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JoVE Journal Genetics
Mediada por CRISPR reorganización de la estructura de bucles de cromatina
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Mediada por CRISPR reorganización de la estructura de bucles de cromatina

Article DOI: 10.3791/57457-v • 09:20 min • September 14th, 2018
September 14th, 2018 •
*1,2, *1, 1, 3, 4, 5, 1, 1, 1, 5, 3, 1,2,6
1Department of Dermatology, Program in Epithelial Biology, Stanford University School of Medicine, 2Program in Cancer Biology, Stanford University School of Medicine, 3Canary Center for Cancer Early Detection, Department of Radiology, Stanford University School of Medicine, 4Department of Biology, Bridgewater State University, 5System Biosciences, 6Veterans Affairs Healthcare System
* These authors contributed equally

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Bucles de cromatina juega un papel importante en la regulación de los genes; sin embargo, ha habido no hay avances tecnológicos que permiten la modificación selectiva y reversible de los bucles de cromatina. Aquí describimos un sistema de gran alcance para la reorganización de bucles de cromatina usando CRISPR-dCas9 (CLOuD9), demostró de forma selectiva y reversible modular la expresión génica en blanco loci.

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Genética número 139 bucles de cromatina plegable dCas9 de expresión CRISPR gen cromatina Regulación génica dimerización reversible dinámico cambio cromosómico.
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