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Journal / Cancer Research / Immunophenotyping av Orthotopic Homograft…
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Cancer Research

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JoVE Journal Cancer Research
Immunophenotyping av Orthotopic Homograft (Syngeneic) av Murine primære KPC bukspyttkjertelen Ductal Adenocarcinoma av flowcytometri
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Immunophenotyping av Orthotopic Homograft (Syngeneic) av Murine primære KPC bukspyttkjertelen Ductal Adenocarcinoma av flowcytometri

Article DOI: 10.3791/57460-v • 08:30 min • October 9th, 2018
October 9th, 2018 •
*1,2, *1, 1, 1, 1, 1, 2, 1,2
1Crown Bioscience Inc., 2State Key Laboratory of Natural and Biomimetic Drugs, Peking University
* These authors contributed equally

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Den eksperimentelle prosedyren på immunophenotyping av murine orthotopic PDAC homografts tar sikte på profilering svulst immuno-microenvironment. Tumorer er orthotopically implantert via kirurgi. Svulster 200-600 mm3 størrelse var høstet og avstand for å forberede encellede suspensjoner, etterfulgt av flere immun markør FACS analyse ved hjelp av ulike fluorescently merket antistoffer.

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Kreftforskning problemet 140 dyr svulst modell Immuno-onkologi Tumor transplantasjon Tumor vev dissosiasjon FACS TIL TAM PDAC
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