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Journal / Cancer Research / Immunophenotyping van Orthotopic Homograft…
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Cancer Research

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JoVE Journal Cancer Research
Immunophenotyping van Orthotopic Homograft (Syngeneic) van de alvleesklier ductaal adenocarcinoom lymfkliertest primaire KPC door Stroom Cytometry
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Immunophenotyping van Orthotopic Homograft (Syngeneic) van de alvleesklier ductaal adenocarcinoom lymfkliertest primaire KPC door Stroom Cytometry

Article DOI: 10.3791/57460-v • 08:30 min • October 9th, 2018
October 9th, 2018 •
*1,2, *1, 1, 1, 1, 1, 2, 1,2
1Crown Bioscience Inc., 2State Key Laboratory of Natural and Biomimetic Drugs, Peking University
* These authors contributed equally

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De experimentele procedure op de immunophenotyping van lymfkliertest orthotopic PDAC homografts is gericht op de profilering van de tumor immuno-communicatie. Tumoren zijn orthotopically geïmplanteerd via chirurgie. Tumoren van 200 – 600 mm3 in grootte werden geoogst en losgekoppeld om te bereiden eencellige schorsingen, gevolgd door multi immuun marker FACS analyse met behulp van verschillende fluorescently gelabelde antilichamen.

Tags

Kankeronderzoek kwestie 140 dierlijke Tumor Model Immuno-oncologie Tumor transplantatie Tumor weefsel dissociatie FACS TIL TAM PDAC
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