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Journal / Cancer Research / Immunophenotyping de Orthotopic la homoplastia…
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JoVE Journal Cancer Research
Immunophenotyping de Orthotopic la homoplastia (Syngeneic) de Adenocarcinoma Ductal pancreática KPC primaria murinas por citometría de flujo
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Immunophenotyping de Orthotopic la homoplastia (Syngeneic) de Adenocarcinoma Ductal pancreática KPC primaria murinas por citometría de flujo

Article DOI: 10.3791/57460-v • 08:30 min • October 9th, 2018
October 9th, 2018 •
*1,2, *1, 1, 1, 1, 1, 2, 1,2
1Crown Bioscience Inc., 2State Key Laboratory of Natural and Biomimetic Drugs, Peking University
* These authors contributed equally

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El procedimiento experimental en el immunophenotyping de homoinjertos PDAC Murino ortotópico pretende perfilar el inmuno-microambiente tumoral. Los tumores son orthotopically implantado mediante cirugía. Tumores de 200 – 600 mm3 en tamaño fueron cosechados y disociados para preparar suspensiones de unicelular, seguidos del análisis de marcador múltiple inmunológico FACS utilizando diferentes anticuerpos marcados con fluorescencia.

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Investigación del cáncer número 140 modelo Animal Tumor inmuno-Oncología trasplante de Tumor Tumor tejido disociación FACS TIL TAM PDAC
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