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Immunology and Infection

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JoVE Journal Immunology and Infection
Påvisning af Inflammasome aktivering og Pyroptotic celledød i Murine knoglemarv-afledte makrofager
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Påvisning af Inflammasome aktivering og Pyroptotic celledød i Murine knoglemarv-afledte makrofager

Article DOI: 10.3791/57463-v • 06:52 min • May 21st, 2018
May 21st, 2018 •
1, 1
1Department of Laboratory Medicine, University of Washington

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Vi beskriver påvisning af NLRP3 inflammasome aktivering på cellulære grundlag ved hjælp af Fluorescens mikroskopi og farvning for aktive caspase-1 og adapteren, ASC. En laktat dehydrogenase release assay er præsenteret for at opdage pyroptotic lysis på grundlag af befolkningen. Disse teknikker kan tilpasses for at studere mange aspekter af inflammasome biologi.

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Immunologi og infektion spørgsmålet 135 immunologi medfødt immunitet inflammasome caspase-1 ASC NLRP3 pyroptosis nigericin murint knoglemarv-afledte makrofager
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