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Journal / Biology / Brug af Anti-phospho-girdin antistoffer til at…
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Biology

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JoVE Journal Biology
Brug af Anti-phospho-girdin antistoffer til at visualisere Intestinal Tuft celler i frit svævende musen Jejunum snit frosset organmateriale
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Brug af Anti-phospho-girdin antistoffer til at visualisere Intestinal Tuft celler i frit svævende musen Jejunum snit frosset organmateriale

Article DOI: 10.3791/57475-v • 06:26 min • March 21st, 2018
March 21st, 2018 •
1, 2, 1, 3, 1, 1, 3, 1,3
1Division of Perinatology, Institute for Developmental Research, Aichi Human Service Center, 2Surgery Department, Anjo Kosei Hospital, 3Department of Pathology, Nagoya University Graduate School of Medicine

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Kuga et al. opdagede at fosforylering-status specifikke antistoffer mod den actin bindende protein girdin fosforyleret på tyrosin 1798 (pY1798) kan bruges til at mærke tuft celler (TCs). Denne protokol giver mulighed for robust visualisering af FKS bruger immunfluorescent farvning af frit svævende jejunum snit frosset organmateriale med pY1798 antistoffer.

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Biologi sag 133 TOT celler antistoffer mod Girdin fosforyleret på tyrosin 1798 Phalloidin gelatine-fyldt tarmen frit svævende Cryosection lav temperatur Antigen hentning
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