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Biology

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Bruk av Anti-phospho-girdin antistoffer visualisere Intestinal dusk celler i frittflytende musen Jejunum Cryosections
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Bruk av Anti-phospho-girdin antistoffer visualisere Intestinal dusk celler i frittflytende musen Jejunum Cryosections

Article DOI: 10.3791/57475-v • 06:26 min • March 21st, 2018
March 21st, 2018 •
1, 2, 1, 3, 1, 1, 3, 1,3
1Division of Perinatology, Institute for Developmental Research, Aichi Human Service Center, 2Surgery Department, Anjo Kosei Hospital, 3Department of Pathology, Nagoya University Graduate School of Medicine

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Mondeo et al. oppdaget at fosforylering status spesifikke antistoffer mot utgangen bindende protein girdin fosforylert på tyrosin 1798 (pY1798) kan brukes til å merke dusk celler (TCs). Denne protokollen lar robust visualisering av TCs bruke immunofluorescent farging av frittflytende jejunum cryosections med pY1798 antistoffer.

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Biologi problemet 133 dusk celler antistoffer mot Girdin fosforylert på tyrosin 1798 Phalloidin Gelatin-fylt tarmen frittflytende Cryosection lav temperatur Antigen henting
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