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Uso de anticuerpos Anti-phospho-girdin visualizar células de penacho Intestinal en Cryosections de yeyuno ratón flotante
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Uso de anticuerpos Anti-phospho-girdin visualizar células de penacho Intestinal en Cryosections de yeyuno ratón flotante

Article DOI: 10.3791/57475-v • 06:26 min • March 21st, 2018
March 21st, 2018 •
1, 2, 1, 3, 1, 1, 3, 1,3
1Division of Perinatology, Institute for Developmental Research, Aichi Human Service Center, 2Surgery Department, Anjo Kosei Hospital, 3Department of Pathology, Nagoya University Graduate School of Medicine

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Kuga et al descubrieron que los anticuerpos específicos de fosforilación-estado contra la actina Unión proteína girdin fosforiladas en tirosina 1798 (pY1798) pueden utilizarse para identificar células de penacho (TCs). Este protocolo permite la visualización sólida de TCs con tinción inmunofluorescente de yeyuno flotante cryosections con anticuerpos pY1798.

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Biología número 133 del penacho de células anticuerpos contra Girdin fosforiladas en tirosina 1798 Phalloidin criosección del intestino flotante lleno de gelatina la recuperación de antígeno de baja temperatura
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