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Journal / Genetics / 基因靶向随机诱变在裂变酵母中选择染色质稳定蛋白酶突变体…
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基因靶向随机诱变在裂变酵母中选择染色质稳定蛋白酶突变体
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基因靶向随机诱变在裂变酵母中选择染色质稳定蛋白酶突变体

Article DOI: 10.3791/57499-v • 07:18 min • May 15th, 2018
May 15th, 2018 •
1, 1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology

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本文介绍了一种用于裂变酵母靶基因随机诱变的详细方法。例如, 我们针对的是rpt4+, 它对19S 蛋白酶体的亚基进行编码, 并筛选出破坏染色质的突变。

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遗传学 问题 135 随机突变 裂变酵母 染色质 易出错 PCR 着丝粒 基因靶向
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