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Journal / Genetics / Gen gezielt Random Mutagenesis Heterochromatin…
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JoVE Journal Genetics
Gen gezielt Random Mutagenesis Heterochromatin destabilisieren Proteasom Mutanten in Spalthefe auswählen
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Gen gezielt Random Mutagenesis Heterochromatin destabilisieren Proteasom Mutanten in Spalthefe auswählen

Article DOI: 10.3791/57499-v • 07:18 min • May 15th, 2018
May 15th, 2018 •
1, 1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology

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Dieser Artikel beschreibt eine detaillierte Methode für zufällige Mutagenese Zielgen in Spalthefe. Als Beispiel Ziel ist es, rpt4 +, die kodiert eine Untereinheit der 19 s Proteasom und Bildschirm für Mutationen, die Heterochromatin destabilisieren.

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Genetik Ausgabe 135 Random Mutagenesis Spalthefe Heterochromatin fehleranfällige PCR Zentromer Gene targeting
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