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Journal / Genetics / Fisyon mayası Heterochromatin kararsız hale…
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JoVE Journal Genetics
Fisyon mayası Heterochromatin kararsız hale Proteasome mutantlar seçmek için rasgele Mutagenesis Gene hedefli
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Fisyon mayası Heterochromatin kararsız hale Proteasome mutantlar seçmek için rasgele Mutagenesis Gene hedefli

Article DOI: 10.3791/57499-v • 07:18 min • May 15th, 2018
May 15th, 2018 •
1, 1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology

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Bu makalede ayrıntılı bir metodoloji fisyon mayası bir hedef gen rasgele mutagenesis için. Örnek olarak, biz bir alt birim 19S proteasome ve ekran heterochromatin istikrarsızlaştırmak mutasyonlar için kodlar rpt4 +, hedef.

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Genetik sorunu 135 rastgele mutagenesis fisyon mayası heterochromatin hataya PCR şeması gen hedefleme
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