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Journal / Biochemistry / Erzeugung von nativen, unmarkierten Huntingtin…
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Biochemistry

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JoVE Journal Biochemistry
Erzeugung von nativen, unmarkierten Huntingtin Exon1 Monomer und Fibrillen mit einem SUMO-Fusion-Strategie
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
Journal (Biochemistry)
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PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
Journal (Biochemistry)
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Erzeugung von nativen, unmarkierten Huntingtin Exon1 Monomer und Fibrillen mit einem SUMO-Fusion-Strategie

Article DOI: 10.3791/57506-v • 11:22 min • June 27th, 2018
June 27th, 2018 •
1, 1, 1, 1
1Laboratory of Molecular and Chemical Biology of Neurodegeneration, Ecole Polytechnique Fédérale de Lausanne (EPFL)

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Hier präsentieren wir Ihnen eine robuste und optimierte Protokoll zur Herstellung von Milligramm-Mengen von native, Tag-freie Monomere und Fibrillen von der exon1 des Huntingtin-Proteins (Httex1), basierend auf die vorübergehende Verschmelzung von kleinen Ubiquitin Verwandte Modifikator (SUMO).

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Biochemie Ausgabe 136 HD Chorea Huntington Huntingtin Neurodegeneration Polyglutamin- PolyQ Aggregation Fibrillen SUMO-Fusion-Technologie Proteinexpression und Reinigung
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