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Journal / Biochemistry / Generation af indfødte, ukodede Huntingtin Exon1…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Generation af indfødte, ukodede Huntingtin Exon1 monomere og fibriller ved hjælp af en SUMO Fusion strategi
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Generation af indfødte, ukodede Huntingtin Exon1 monomere og fibriller ved hjælp af en SUMO Fusion strategi

Article DOI: 10.3791/57506-v • 11:22 min • June 27th, 2018
June 27th, 2018 •
1, 1, 1, 1
1Laboratory of Molecular and Chemical Biology of Neurodegeneration, Ecole Polytechnique Fédérale de Lausanne (EPFL)

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Vi præsenterer her, en robust og optimeret protokol for produktion af milligram mængder af indfødte, tag-gratis monomerer og fibriller af exon1 af Huntingtin-protein (Httex1) baseret på den forbigående fusion af små ubiquitin relaterede modifier (SUMO).

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Biokemi sag 136 HD Huntington's chorea huntingtin neurodegeneration polyglutamin polyQ sammenlægning fibriller SUMO fusionsteknologi protein proteinekspression og -oprensning
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