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Journal / Biochemistry / ネイティブ、タグなしの Huntingtin Exon1…
JoVE Journal
Biochemistry

This content is Open Access.

JoVE Journal Biochemistry
ネイティブ、タグなしの Huntingtin Exon1 モノマーと相撲の融合戦略を使用して線維の生成
An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
Journal (Biochemistry)
An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins
Journal (Biochemistry)
Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins
 
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ネイティブ、タグなしの Huntingtin Exon1 モノマーと相撲の融合戦略を使用して線維の生成

Article DOI: 10.3791/57506-v • 11:22 min • June 27th, 2018
June 27th, 2018 •
1, 1, 1, 1
1Laboratory of Molecular and Chemical Biology of Neurodegeneration, Ecole Polytechnique Fédérale de Lausanne (EPFL)

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ここでは、ネイティブなタグ無料モノマーのミリグラム量と小さなユビキチン関連修飾子 (相撲) の一時的な融合に基づくハンチンチン蛋白質 (Httex1) の exon1 の線維の生産のための堅牢な最適化されたプロトコルを提案する.

Tags

生化学、問題 136、HD、ハンチントン病、huntingtin、神経変性、ポリグルタミン、polyQ、集約、線維、相撲融合技術、蛋白質の表現および浄化
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