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JoVE Journal Immunology and Infection
La Lambda seleccione cII sistema de detección de mutación
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La Lambda seleccione cII sistema de detección de mutación

Article DOI: 10.3791/57510-v • 07:08 min • April 26th, 2018
April 26th, 2018 •
1, 1
1Department of Preventive Medicine, USC Keck School of Medicine, University of Southern California

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Se describe un protocolo detallado para el ensayo de mutación Lambda seleccione cII en cultivos de células de roedores transgénicos o los correspondientes animales tratados con un agente químico físico de interés. Este enfoque ha sido ampliamente utilizado para las pruebas de mutagenicidad de agentes carcinógenos en células de mamíferos.

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Inmunología e infección número 134 bacteriófago cII transgen fibroblastos embrionarios de ratón EMF mutación Vector de la lanzadera
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