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En hurtig og letkøbt Pipeline for generering af genomisk punkt mutanter i C. elegans bruger CRISPR/Cas9 Ribonucleoproteins
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Genetics
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JoVE Journal Genetics
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

En hurtig og letkøbt Pipeline for generering af genomisk punkt mutanter i C. elegans bruger CRISPR/Cas9 Ribonucleoproteins

English

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7,397 Views

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08:37 min

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April 30, 2018

DOI:

10.3791/57518-v

DOI:
10.3791/57518-v

•

08:37 min
April 30, 2018

•

3 Views
, , , ,
1Department of Pharmacology,University of Michigan

Summary

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Vi præsenterer her, en metode til at ingeniør genomet af C. elegans ved hjælp af CRISPR-Cas9 ribonucleoproteins og homologi afhængige reparation skabeloner.

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Cite this Article

Prior, H., MacConnachie, L., Martinez, J. L., Nicholl, G. C., Beg, A. A. A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins. J. Vis. Exp. (134), e57518, doi:10.3791/57518 (2018).

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