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JoVE Journal Biochemistry
Toeprinting analyse de Translation Initiation complexe formé sur l’ARNm chez les mammifères
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Toeprinting analyse de Translation Initiation complexe formé sur l’ARNm chez les mammifères

Article DOI: 10.3791/57519-v • 10:37 min • May 10th, 2018
May 10th, 2018 •
1, 1,2,3
1Department of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, 2Department of Neuroscience and the Canadian Centre for Behavioral Neuroscience (CCBN), University of Lethbridge, 3Arnie Charbonneau Cancer Institute, University of Calgary

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Toeprinting a pour but de mesurer la capacité de in vitro transcrit RNA pour former des complexes initiation traduction de ribosomes dans une variété de conditions. Ce protocole décrit une méthode pour toeprinting ARN chez les mammifères et peut être utilisé pour étudier la traduction fois cap-dépendante et pilotée par l’IRES.

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Numéro 135 Toeprinting règlement de translation biochimie initiation de la traduction IRES cellulaires 5' cap facteurs d’initiation eucaryote chapeau-indépendante ARNm SIFE
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