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JoVE Journal Cancer Research
Livraison de virus de CRISPR Guides pour la Prostate Murine pour modifications géniques
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Livraison de virus de CRISPR Guides pour la Prostate Murine pour modifications géniques

Article DOI: 10.3791/57525-v • 06:21 min • April 27th, 2018
April 27th, 2018 •
1, 1, 2, 2, 1
1Department of Clinical Medicine, Aarhus University, 2GDD, Cancer Cell Biology Program, National Cancer Research Center (CNIO)

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Ce protocole décrit une méthode nouvellement créée pour la livraison de virus à la prostate murine. Utilisant la technologie CRISPR/Cas9, surexpression du gène ou livraison recombinase Cre, la technique permet l’orthotopic altération de l’expression génique et implémente un modèle de souris roman pour cancer de la prostate.

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Recherche sur le cancer numéro 134 cancer de la Prostate génome édition thérapie génique modifications géniques livraison de virus injection de virus virus adeno-associé à CRISPR/Cas9 modèle tumorale in vivo initiation tumorale
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