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Journal / Neuroscience / 前子宫内胚胎小鼠脑电穿孔和脊髓切片培养迁移 GABAergic…
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Neuroscience

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JoVE Journal Neuroscience
前子宫内胚胎小鼠脑电穿孔和脊髓切片培养迁移 GABAergic 中间神经元的活体成像
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前子宫内胚胎小鼠脑电穿孔和脊髓切片培养迁移 GABAergic 中间神经元的活体成像

Article DOI: 10.3791/57526-v • 09:50 min • April 20th, 2018
April 20th, 2018 •
1,2, 1, 1,3, 1,2,4
1Centre de recherche du CHU Sainte-Justine, 2Department of Neuroscience, Université de Montréal, 3Department of pathology and cellular biology, Université de Montréal, 4Department of Pediatrics, Université de Montréal

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在这里, 我们提供了一个低成本和可靠的方法来产生转脑脊髓切片培养的小鼠胚胎适合共焦显微镜和实时成像技术。

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神经科学 问题 134 大脑发育 共焦显微术 皮质 GABAergic 中间神经元 e13.5 胚 电穿孔 基因抑制 小鼠 神经元迁移 脊髓培养 RNA 干扰 切向迁移 时间推移成像
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