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JoVE Journal Neuroscience
Ex Utero Electroporation og Organotypic stykke kulturer embryonale musen hjerner for Live-Imaging av overføring av GABAergic Interneurons
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Ex Utero Electroporation og Organotypic stykke kulturer embryonale musen hjerner for Live-Imaging av overføring av GABAergic Interneurons

Article DOI: 10.3791/57526-v • 09:50 min • April 20th, 2018
April 20th, 2018 •
1,2, 1, 1,3, 1,2,4
1Centre de recherche du CHU Sainte-Justine, 2Department of Neuroscience, Université de Montréal, 3Department of pathology and cellular biology, Université de Montréal, 4Department of Pediatrics, Université de Montréal

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Her gir vi en rimelig og pålitelig metode for å generere electroporated hjernen organotypic skive kulturer fra mouse embryoer egnet for AC confocal mikroskopi og live-imaging teknikker.

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Nevrovitenskap problemet 134 hjernens utvikling AC confocal mikroskopi kortikale GABAergic interneurons e13.5 embryo electroporation gen undertrykkelse mus neuronal migrasjon organotypic kultur RNA-interferens tangentiell migrasjon time-lapse Imaging
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