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Journal / Neuroscience / Ex Utero Elettroporazione e colture Organotypic…
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Neuroscience

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JoVE Journal Neuroscience
Ex Utero Elettroporazione e colture Organotypic fetta di cervelli embrionali del Mouse per vivere-Imaging di migrazione interneuroni GABAergici
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High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium
A Method for Tracking the Time Evolution of Steady-State Evoked Potentials
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Ex Utero Elettroporazione e colture Organotypic fetta di cervelli embrionali del Mouse per vivere-Imaging di migrazione interneuroni GABAergici

Article DOI: 10.3791/57526-v • 09:50 min • April 20th, 2018
April 20th, 2018 •
1,2, 1, 1,3, 1,2,4
1Centre de recherche du CHU Sainte-Justine, 2Department of Neuroscience, Université de Montréal, 3Department of pathology and cellular biology, Université de Montréal, 4Department of Pediatrics, Université de Montréal

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Qui, forniamo un metodo affidabile e a basso costo per generare colture individulamente cervello organotipiche fetta da embrioni del mouse adatti per la microscopia confocale e tecniche di imaging live.

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Neuroscienze problema 134 lo sviluppo del cervello microscopia confocale corticale interneuroni GABAergici e13.5 embrione elettroporazione repressione genica mouse migrazione neuronale coltura organotypic RNA interference migrazione tangenziale time-lapse di imaging
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