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JoVE Journal Neuroscience
Ex Utero Electroporation en Organotypic Slice culturen van muis embryonale hersenen voor Live-Imaging van GABAergic interneuronen migreren
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Ex Utero Electroporation en Organotypic Slice culturen van muis embryonale hersenen voor Live-Imaging van GABAergic interneuronen migreren

Article DOI: 10.3791/57526-v • 09:50 min • April 20th, 2018
April 20th, 2018 •
1,2, 1, 1,3, 1,2,4
1Centre de recherche du CHU Sainte-Justine, 2Department of Neuroscience, Université de Montréal, 3Department of pathology and cellular biology, Université de Montréal, 4Department of Pediatrics, Université de Montréal

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Hier bieden we een goedkope en betrouwbare methode voor het genereren van electroporated brein organotypic segment culturen van muis embryo's geschikt voor confocale microscopie en live-imaging technieken.

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Neurowetenschappen kwestie 134 ontwikkeling van de hersenen confocal microscopie corticale GABAergic interneuronen e13.5 embryo electroporation gene repressie muis neuronale migratie organotypic cultuur RNA-interferentie tangentiële migratie time-lapse Imaging
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