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Journal / Bioengineering / Antimikrobiella peptider produceras av…
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Bioengineering

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JoVE Journal Bioengineering
Antimikrobiella peptider produceras av selektionstryck införlivandet av icke-kanoniska aminosyror
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Antimikrobiella peptider produceras av selektionstryck införlivandet av icke-kanoniska aminosyror

Article DOI: 10.3791/57551-v • 11:56 min • May 4th, 2018
May 4th, 2018 •
*1, *1, 2, 3, 3, 2, 1
1Department of Biocatalysis, Institute of Chemistry, Technische Universität Berlin, 2Department of Bioenergetics, Institute of Chemistry, Technische Universität Berlin, 3Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Department of Molecular Genetics, University of Groningen
* These authors contributed equally

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Presenteras i protokollet den Escherichia coli-baserat selektionstryck införlivandet av icke-kanoniska aminosyror (ncAAs) i Lactococcus antimikrobiell peptid nisin. Dess egenskaper kan ändras under rekombinant uttryck via substitution med önskad ncAAs i definierade tillväxt medier. Förändringar i bioaktivitet mappas av tillväxt hämning analyser och fluorescensmikroskopi.

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Bioteknik fråga 135 Nisin RiPPs ampere ncAAs lantibiotic prolin analog romanen antibiotika aktivitet assay grampositiva bakterier rekombinant produktion posttranslationell modifiering syntetisk biologi
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