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Journal / Bioengineering / Antimikrobielle peptider produceret af selektiv…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Antimikrobielle peptider produceret af selektiv pres indarbejdelse af ikke-kanoniske aminosyrer
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Antimikrobielle peptider produceret af selektiv pres indarbejdelse af ikke-kanoniske aminosyrer

Article DOI: 10.3791/57551-v • 11:56 min • May 4th, 2018
May 4th, 2018 •
*1, *1, 2, 3, 3, 2, 1
1Department of Biocatalysis, Institute of Chemistry, Technische Universität Berlin, 2Department of Bioenergetics, Institute of Chemistry, Technische Universität Berlin, 3Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Department of Molecular Genetics, University of Groningen
* These authors contributed equally

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Protokollen præsenterer Escherichia coli-baseret selektivt pres indarbejdelse af ikke-kanoniske aminosyrer (ncAAs) i lactococcal antimikrobielle peptid nisin. Dens egenskaber kan ændres under rekombinant udtryk via substitution med ønskede ncAAs i definerede vækst medier. Deraf følgende ændringer i bioactivity er kortlagt af vækst hæmning assays og Fluorescens mikroskopi.

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Bioteknologi spørgsmålet 135 Nisin RiPPs forstærkere ncAAs lantibiotic prolin analog Roman antibiotika aktivitet assay Gram-positive bakterier rekombinant produktion posttranslationelle modifikation syntetisk biologi
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