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Journal / Bioengineering / Antimikrobielle peptider produseres av…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Antimikrobielle peptider produseres av seleksjonspress innlemmelse av ikke-kanoniske aminosyrer
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Antimikrobielle peptider produseres av seleksjonspress innlemmelse av ikke-kanoniske aminosyrer

Article DOI: 10.3791/57551-v • 11:56 min • May 4th, 2018
May 4th, 2018 •
*1, *1, 2, 3, 3, 2, 1
1Department of Biocatalysis, Institute of Chemistry, Technische Universität Berlin, 2Department of Bioenergetics, Institute of Chemistry, Technische Universität Berlin, 3Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Department of Molecular Genetics, University of Groningen
* These authors contributed equally

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Protokollen presenterer Escherichia coli-basert seleksjonspress innlemmelse av ikke-kanoniske aminosyrer (ncAAs) i lactococcal antimikrobielle peptid nisin. Egenskaper kan endres under rekombinant uttrykk via substitusjon med ønsket ncAAs i definerte vekst medier. Resulterende endringer i bioactivity tilordnes av vekst hemming analyser og fluorescens mikroskopi.

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Bioteknologi problemet 135 Nisin RiPPs forsterkere ncAAs lantibiotic proline analog romanen antibiotika aktivitet analysen Gram-positive bakteriene rekombinant produksjon posttranslational endring syntetisk biologi
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