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Journal / Biology / Laser Capture lokalt av högt rent trabekelverket…
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Laser Capture lokalt av högt rent trabekelverket från mus ögon för gen uttryck analys
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Laser Capture lokalt av högt rent trabekelverket från mus ögon för gen uttryck analys

Article DOI: 10.3791/57576-v • 13:47 min • June 3rd, 2018
June 3rd, 2018 •
*1, *2, *1, 2, 2, 2,3, 2,4, 1
1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research, National Institute of Environmental Health Sciences, NIH, 2Cellular and Molecular Pathology Branch, Division of the National Toxicology Program, National Institute of Environmental Health Sciences, NIH, 3Integrated Laboratory Systems Inc., 4Experimental Pathology Laboratories Inc.
* These authors contributed equally

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Här beskriver vi ett protokoll för en reproducerbar laser fånga lokalt (LCM) för att isolera trabekelverket (TM) för nedströms RNA analys. Förmågan att analysera förändringar i genuttryck i Översättningsminnet hjälp att förstå de bakomliggande molekylära mekanismerna för TM-relaterade ögonsjukdomar.

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Biologi fråga 136 glaukom trabekelverket laser fånga lokalt laser lokalt RNA isolering genuttryck LCM LMD
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