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Captação microdissection da malha Trabecular altamente pura de olhos de rato de laser para análise de expressão do Gene
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Captação microdissection da malha Trabecular altamente pura de olhos de rato de laser para análise de expressão do Gene

Article DOI: 10.3791/57576-v • 13:47 min • June 3rd, 2018
June 3rd, 2018 •
*1, *2, *1, 2, 2, 2,3, 2,4, 1
1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research, National Institute of Environmental Health Sciences, NIH, 2Cellular and Molecular Pathology Branch, Division of the National Toxicology Program, National Institute of Environmental Health Sciences, NIH, 3Integrated Laboratory Systems Inc., 4Experimental Pathology Laboratories Inc.
* These authors contributed equally

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Aqui, descrevemos um protocolo para um laser reprodutíveis captura microdissection (LCM) para isolar a malha trabecular (TM) para a análise de RNA a jusante. A capacidade de analisar as alterações na expressão gênica na TM vai ajudar na compreensão do mecanismo molecular subjacente de doenças oculares relacionadas ao TM.

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Biologia questão 136 Glaucoma malha trabecular microdissection de captura do laser laser microdissection isolamento de RNA expressão gênica LCM LMD
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