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Developmental Biology

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Hele Mount immunofluorescens og follikel kvantificering af kulturperler mus æggestokke
 
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Hele Mount immunofluorescens og follikel kvantificering af kulturperler mus æggestokke

Article doi: 10.3791/57593
May 2nd, 2018

Summary May 2nd, 2018

Please note that all translations are automatically generated.

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Vi præsenterer her, en protokol for at kvantificere follikler i kulturperler æggestokkene af unge mus uden seriel skæring. Brug hele orgel immunofluorescens og væv clearing, er fysiske skæring erstattet med optisk skæring. Denne metode til forberedelse af prøver og visualisering fastholder orgel integritet og letter automatiserede kvantificering af specifikke celler.

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