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Journal / Developmental Biology / Hele Mount immunofluorescens og follikel…
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JoVE Journal Developmental Biology
Hele Mount immunofluorescens og follikel kvantificering af kulturperler mus æggestokke
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Hele Mount immunofluorescens og follikel kvantificering af kulturperler mus æggestokke

Article DOI: 10.3791/57593-v • 08:15 min • May 2nd, 2018
May 2nd, 2018 •
1, 2, 1,2
1Department of Biomedical Sciences, Cornell University, 2Department of Molecular Biology and Genetics, Cornell University

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Vi præsenterer her, en protokol for at kvantificere follikler i kulturperler æggestokkene af unge mus uden seriel skæring. Brug hele orgel immunofluorescens og væv clearing, er fysiske skæring erstattet med optisk skæring. Denne metode til forberedelse af prøver og visualisering fastholder orgel integritet og letter automatiserede kvantificering af specifikke celler.

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Udviklingsmæssige biologi spørgsmålet 135 reproduktiv biologi Mus musculus æggestok kultur væv clearing hele mount imaging immunofluorescens
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