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Journal / Neuroscience / 膜片钳后单细胞多重反转录聚合酶链反应…
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JoVE Journal Neuroscience
膜片钳后单细胞多重反转录聚合酶链反应
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膜片钳后单细胞多重反转录聚合酶链反应

Article DOI: 10.3791/57627-v • 10:44 min • June 20th, 2018
June 20th, 2018 •
*1, *1, *1, 1
1UPMC Univ Paris 06, INSERM, CNRS, Neuroscience Paris Seine - Institut de Biologie Paris Seine (NPS - IBPS), Sorbonne Université
* These authors contributed equally

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本协议描述了在膜片钳后执行单细胞多重反向转录聚合酶链反应所需的关键步骤和预防措施。该技术是一种简单有效的方法, 用于分析由膜片钳记录的单个细胞所确定的一组基因的表达谱。

Tags

神经科学 问题 136 基因表达谱 神经元 星形胶质细胞 脑切片 全细胞构型 嵌套聚合酶链反应 (PCR)
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