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Aislamiento de las células gliales entéricas de la Submucosa y la lámina propia del ratón adulto
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Aislamiento de las células gliales entéricas de la Submucosa y la lámina propia del ratón adulto

Article DOI: 10.3791/57629-v • 07:53 min • August 15th, 2018
August 15th, 2018 •
1,4, 1, 1, 1, 1, 3, 1,2, 1,2,5
1Department of Internal Medicine-Gastroenterology, University of Michigan, 2Department of Molecular and Integrative Physiology, University of Michigan, 3Department of Molecular, Cellular and Developmental Biology, University of Michigan, 4Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University, 5Division of Gastroenterology, University of Arizona College of Medicine

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Aquí, describimos el aislamiento de las células entéricas glial de la intestinal submucosa con incubaciones secuenciales de EDTA para quelar cationes divalentes y luego de incubación en la solución de recuperación no enzimática de la célula. La suspensión de células resultante de poly-D-lisina y laminina de la galjanoplastia resulta en una cultura altamente enriquecida de las células gliales submucosas de análisis funcional.

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Biología número 138 duodeno hGFAP-Cre tdTomato quelación EDTA EGCs Ca2 + flujo Sox10 GFAP
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